LAB ASSISTANT
Name of Sector Medical & Nursing Name of Module Lab Assistant
Entry Qualifications 10th Standard + 18 years and above+ MED102 (basics of anatomy and physiology) Duration 450 Hrs
Contents Pathology, Microbilogy And Bid-chemistry

After completing the training the candidate will be able to assist the Technician & Pathologist in the Lab.



Practical Competencies Underpinning Knowledge (Theory)

Pathology (140 Hrs)

  • Techniques and labeling of the sample Preparation of anticoagulants
  • RBC, WBC, platelet count, CBC and complete Haemograme.
  • ESR stands & ESR estimation
  • PCV & calculation of RBC indices
  • Urine - Physical Examination & Chemical Examination


  • Blood Collection procedure
  • Precaution and smearing
  • Chemicals used in different types of tests.
  • Hb estimation by different methods
  • Stool, Biological fluids examination.
  • Knowledge on Vacotainer.

MICROBILOGY (150 Hrs)

  • Cleaning and care of glassware, syringes, apparatus, preparation of Pasteur Pipettes.
  • Handling and care of Microscope
  • Operation of Autoclave, Incubator, waterbath, seitzfilter
  • Preparation of various Media
  • Preparation of stains and smears


  • Lab instructions for Personal Safety precaution'
  • Receipt and recording a specimen in the lab and dispatch of specimen
  • Methods of collection of microbiology specimen-and its importance and processing.

BID-CHEMISTRY (160 Hrs)

  • Collection of Capillary blood
  • Collection of Venous blood
  • Separation of Serum from clotted blood
  • Separation of plasma from blood
  • Preparation of protein free blood filtrate
  • Lab glass ware
  • i. Identification

    ii. Handling

    iii. Care and Maintenance

    iv. Uses

  • Lab instruments
  • i. Centrifuges

    ii. Balances

    iii. Photo Electric colorimeter

    iv. Spectrophotometer

  • Preparation of
  • i. Percentage solutions

    ii. Normal solutions



  • Reception and registration procedure.
  • Understanding of blood related terms
  • i. Capillary blood

    ii. Venous blood

    iii. Serum, Plasma, Protein

    iv. Clotted blood

  • Collection procedures of Blood
  • Knowledge on different types of lab instruments.
  • Qualitative identification tests of sugars
  • Qualitative identification tests of proteins
  • Quantitative determination of Blood sugar
  • Glucose Tolerance test
  • Quantitative determination of Blood urea
  • Quantitative determination of Serum creatinine
  • Quantitative determination of Urine Sugar